By Dr. Ashok Kumar Dr. Ashok Bendre
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The purpose of Plant Proteomics: tools and Protocols is to offer up-- date equipment and protocols utilized by well-known scientists on the planet of plant proteomics. If this international used to be a really small one twenty-five years in the past while the 1st papers have been released, it has given that skilled exponential progress, and in so much international locations worldwide there are laboratories engaged on plant proteomics.
Great advances were made in concepts and alertness of microscopy because the authors' unique booklet of Plant telephone Biology, An Ultrastructural technique in 1975. With this revision, the authors have additional over two hundred photographs exploiting sleek concepts similar to cryo-microscopy, immuno-gold localisations, immunofluorescence and confocal microscopy, and in situ hybridisation.
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2. Filaments are heterotrichous and differentiated into (i) prostrate portion and (ii) an erect portion. 3. In some species prostrate portion is irregularly and profusely branched or altogether absent. If present it remains attached to the Fig. 2. Ectocarpus. A part of the fIlamentous thallus. " ,' . Algae ",' 5. The branches and the main axis are uniseriate. 6. The erect branches have intercalary meristem just below the terminal hair. It results in trichothallic growth. 7. The cells are squarish to cylindrical and uninucleate.
3. It is a thick walled structure being made of 3-7 layers. 4. The protoplast of a zygote is very dense. ,~,.. ~--receptive pore (beak) oogonial wall zygote branch filament c Fig. 2. Vaucheria. A-D. Various stages in sexual reproduction. A. Young oogonium with young coiled aniheridium, B. Mature oogonium and antheridium, C. Stalked sex organs in Vaucheria geminata, D. Empty antheridium and zygote. 5. Numerous oil droplets are scattered throughout the protoplasm. 6. It is considered to be a diploid structure, as such there is said to be possibility of zygotic meiosis.
7. 8. 9. Oogonium generally shows one or more cap cells at its upper end, indicating its development from a comparatively older cell. It is mostly spherical or oval in shape and larger than a vegetative cell. At the base of each oogonium lies a small and flat daughter cell, known as supporting or suffultory cell. Oogonium encloses a single large ovum. The wall of the oogonium has a small pore on one side, Known as receptive pore. Just opposite the receptive pore, protoplast of Il~c oogonium has a hyaline area-receptive spot.
A text book of practical botany 1 by Dr. Ashok Kumar Dr. Ashok Bendre
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